Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Structural changes in the lacrimal sac epithelium and associated lymphoid tissue during experimental dacryocystitis

doi: 10.2147/OPTH.S26048

Figure Lengend Snippet: Immunofluorescent micrographs showing colocalization of CD20-positive B lymphocytes and CD3-positive T lymphocytes in the control ( A – C ) and inflamed lacrimal sac 1 month ( D – F ) and 3 months ( G – I ) after Staphylococcus aureus inoculation. In control tissue, a small number of CD20-positive ( A , red) and CD3-positive lymphocytes ( B , green) were codistributed ( C ) in the lamina propria. One month after inoculation, numerous CD20-positive ( D , red) and CD3-positive lymphocytes ( E , green) were coinfiltrated ( F ) into the lamina propria. Three months after inoculation, the numbers of CD20-positive ( G , red) and CD3-positive ( H , green) lymphocytes codistributed ( I ) were decreased compared with 1 month. Notes: Bars = 65 μm; blue, diamidino-2-phenylindole-stained nuclei.

Article Snippet: The same sections were also incubated with rabbit antihuman CD3 antibody (1:100; Sigma-Aldrich, St Louis, MO) to colocalize T lymphocytes.

Techniques: Staining

Journal: Toxicologic Pathology

Article Title: Impact of Preanalytical Factors During Histology Processing on Section Suitability for Digital Image Analysis

doi: 10.1177/0192623320970534

Figure Lengend Snippet: Reagents for IHC Staining.

Article Snippet: Detection of CD3 was carried out (after HIER with TRS, high pH) using a rabbit polyclonal antihuman CD3 antibody (Dako, Cat No. A0452).

Techniques: Staining, Concentration Assay, Incubation

Journal: Toxicologic Pathology

Article Title: Impact of Preanalytical Factors During Histology Processing on Section Suitability for Digital Image Analysis

doi: 10.1177/0192623320970534

Figure Lengend Snippet: The staining intensity of a particular IHC method is influenced more by the biomarker than the choice of stainer. Data are shown as the CV (calculated as the SD divided by the mean) for the average OD within each staining run for each biomarker on a given instrument for all tissues. The box plots represent the distribution of the data, where the box defines the range encompassing values between the 25th and 75th quartiles, the line within the box is drawn at the median OD, and the whiskers demonstrate the expected variation in the data. Dots located over some boxes (eg, CD3 on instrument 0083) plot data that fell beyond the whiskers, which are indicative of staining runs with higher than expected variability. For Ki-67, the expanded ranges (longer boxes) on all 3 stainers denote that staining was more variable across runs for sections containing lymphoid tissue (see ). Instrument models: AutostainerPlus Link, Nos. 0010 and 0083; Autostainer Link 48, No. 0151. CV indicates coefficient of variation; DAB, 3,3′-diaminobenzidene; IHC, immunohistochemical; OD, optical density; SD, standard deviation.

Article Snippet: Detection of CD3 was carried out (after HIER with TRS, high pH) using a rabbit polyclonal antihuman CD3 antibody (Dako, Cat No. A0452).

Techniques: Staining, Biomarker Assay, Immunohistochemical staining, Standard Deviation

Journal: Toxicologic Pathology

Article Title: Impact of Preanalytical Factors During Histology Processing on Section Suitability for Digital Image Analysis

doi: 10.1177/0192623320970534

Figure Lengend Snippet: Staining intensity may be impacted unexpectedly by inadvertent adjustments to the IHC protocol design. In this example, Ki-67-positive cells in 4-µm-thick step sections exhibit stronger staining when processed using a protocol in which the incubation in primary antibody is followed rapidly by application of the visualization reagent (ie, the standard protocol [column A]) relative to 4-µm thick step sections in which the primary antibody was followed by a 35-min incubation in buffer before addition of the visualization reagent (column B); the extended buffer incubation for Ki-67 corresponded to the time needed to accommodate another biomarker in the same staining run (CD45, which requires incubation with a secondary antibody prior to application of the visualization reagent). The decreased staining intensity associated with extended rinsing for some staining runs likely explains the higher variation in Ki-67 labeling across instruments (see ) relative to labeling provided by the other biomarkers tested in this study (CD3, CD45, F4/80). IHC indicates immunohistochemical.

Article Snippet: Detection of CD3 was carried out (after HIER with TRS, high pH) using a rabbit polyclonal antihuman CD3 antibody (Dako, Cat No. A0452).

Techniques: Staining, Incubation, Biomarker Assay, Labeling, Immunohistochemical staining

Journal: JNCI Cancer Spectrum

Article Title: Contribution of Immunoscore and Molecular Features to Survival Prediction in Stage III Colon Cancer

doi: 10.1093/jncics/pkaa023

Figure Lengend Snippet: Representative hematoxylin-stained tissue sections showing CD3 + and CD8 + T cells and CD20 + B cells in stage III human colon cancers. The core tumor (CT) and the invasive margin (IM) are shown with the demarcation of the IM ( red line ; see "Methods") separating malignant glands/cells from peritumoral stroma.

Article Snippet: Sections were incubated at 37°C with primary antibodies: rabbit monoclonal antihuman CD3 (MRQ-39, Cell Marque, Rocklin, CA), mouse antihuman monoclonal antibodies against CD8 + (C8/144B, Cell Marque), and CD20 + (MO755, clone L26, DAKO).

Techniques: Staining

Journal: JNCI Cancer Spectrum

Article Title: Contribution of Immunoscore and Molecular Features to Survival Prediction in Stage III Colon Cancer

doi: 10.1093/jncics/pkaa023

Figure Lengend Snippet: Univariate associations of clinical, immune, and molecular features (in best functional form) with DFS

Article Snippet: Sections were incubated at 37°C with primary antibodies: rabbit monoclonal antihuman CD3 (MRQ-39, Cell Marque, Rocklin, CA), mouse antihuman monoclonal antibodies against CD8 + (C8/144B, Cell Marque), and CD20 + (MO755, clone L26, DAKO).

Techniques: Functional Assay